Comparison of various decalcificators in preparation of DNA from human rib bones

Magdalena Żołędziewska; Stanisław Gronkiewicz; Tadeusz Dobosz

doi:10.18778/1898-6773.65.07

Authors

Magdalena Żołędziewska Institute of Forensic Medicine, Medical University, ul. Mikulicza- Radeckiego 4, 50-368 Wrocław, Poland
Stanisław Gronkiewicz Institute of Anthropology, Polish Academy of Sciences, ul. Kuźnicza 35, 50-951 Wrocław
Tadeusz Dobosz Institute of Forensic Medicine, Medical University, ul. Mikulicza- Radeckiego 4, 50-368 Wrocław, Poland

DOI:

https://doi.org/10.18778/1898-6773.65.07

Keywords:

DNA preparation, decalcification, DNA degradation

Abstract

Various calcium-binding buffers and agents in DNA preparation from fresh human rib bones were compared. We showed that the buffer described by Kuntze et al. [1996] yielded the highest quantities of DNA, but the obtained DNA was strongly degraded. The best quality DNA was obtained using two buffers: of Hänni et al. [1994] and of Ivanov et al. [1995]. Among the new calcium-binding agents that were not examined before, the most efficient were Bismuth III Oxide and Na/FeEDTA, and – in two-step decalcification – sodium citrate.

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