Analysis of indole derivatives in methanolic extracts from mycelium of Agaricus bisporus cultured in vitro on liquid Oddoux medium

Bożena Muszyńska; Katarzyna Sułkowska-Ziaja; Patrycja Hałaszczuk; Remigiusz Krężałek; Maciej Łojewski

doi:10.2478/fobio-2014-0002

Authors

Bożena Muszyńska Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany
Katarzyna Sułkowska-Ziaja Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany
Patrycja Hałaszczuk Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany
Remigiusz Krężałek Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany
Maciej Łojewski Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany

DOI:

https://doi.org/10.2478/fobio-2014-0002

Keywords:

Agaricus bisporus, in vitro culture, L-tryptophan, serotonin

Abstract

Methanolic extracts obtained from biomass of Agaricus bisporus (J.E. Lange) Imbach cultured in vitro were analyzed for qualitative and quantitative composition of non-hallucinogenic indole compounds in order to compare their amount with fruiting bodies of these species. Extracts demonstrated to contain six indole compounds. Contents of individual compounds ranged from 0.01 to 21.33 mg/100 g d.w. in biomass from in vitro cultures. The quantitatively dominating compounds included: 5-hydroxytryptophan (12.50 mg/100 g d.w.), L-tryptophan (14.00 mg/100 g d.w.) and serotonin (7.00 mg/100 g d.w.). Total content of the remaining indole compounds under analysis in the study was 55.32 mg/100 g d.w.

Downloads

Download data is not yet available.

References

Barros, L., Cruz, T., Baptista, P., Estevinho, L.M. & Ferreira, I. 2008. Wild and commercial mushrooms as source of nutrients and nutraceuticals. Food Chemistry and Toxicology, 46: 2742–2747.
Google Scholar

Ey, J., Schömi, E. & Taubert, D. 2007. Dietary sources and antioxidant effects of ergothioneine. Journal of Agricultural and Food Chemistry, 55: 6466–6474.
Google Scholar

Knudsen, H. & Vesterholt, J. 2008. Funga Nordica: agaricoid, boletoid and cyphelloid genera. Copenhagen, Nordsvamp.
Google Scholar

Koyalamudi, S.R., Jeong, S.C., Song, C.H., Cho, K.Y. & Pang, G. 2008. Vitamin D2 formation and bioavailability from Agaricus bisporus button mushrooms treated with ultraviolet irradiation. Journal of Agriculture and Food Chemistry, 57: 3351–3355.
Google Scholar

Kysilka, R. & Wurst, M. 1985. High performance liquid chromatographic determination of hallucinogenic indoleamins with simultaneous UV photometric and voltametric detection. Journal of Chromatography, 320: 414–420.
Google Scholar

Muszyńska, B. & Sułkowska-Ziaja, K. 2012a. Analysis of indole compounds in edible Basidiomycota species after thermal processing. Food Chemistry, 132: 455–459.
Google Scholar

Muszyńska, B. & Sułkowska-Ziaja, K. 2012b. Analysis of indole compounds in fruiting bodies and in mycelia from in vitro cultures of Calocera viscosa (Basidiomycota). Acta Mycologica, 47: 57–64.
Google Scholar

Muszyńska, B., Maślanka, A., Sułkowska-Ziaja, K. & Krzek, J. 2007. Analysis of indole compounds and nitric bases in fruiting bodies in Lactarius deterrimus by TLC-UV. Journal of Planar Chromatography Modern TLC, 20: 55–58.
Google Scholar

Muszyńska, B., Sułkowska-Ziaja, K. & Ekiert, H. 2009. Indole compounds in fruiting bodies of some selected Macromycetes species and in their mycelia cultured in vitro. Die Pharmazie, 64: 479–480.
Google Scholar

Muszyńska, B., Sułkowska-Ziaja, K. & Ekiert, H. 2011a. Indole compounds in fruiting bodies of some edible Basidiomycota species. Food Chemistry, 125: 1306–1308.
Google Scholar

Muszyńska, B., Sułkowska-Ziaja, K. & Ekiert, H. 2011b. Indole compounds in some culinary– medicinal higher basidiomycetes from Poland. International Journal of Medicinal Mushrooms, 13: 449–454.
Google Scholar

Muszyńska, B., Maślanka, A., Sułkowska-Ziaja, K. & Ekiert H. 2011c. Analysis of indole compounds in Armillaria mellea fruiting bodies. Acta Poloniae Pharmaceutica – Drug Research, 68: 93–97.
Google Scholar

Oddoux, L. (ed.). 1957. Recherches sur les mycéliums secondaires des Homobasidiés en culture pure. Imprimerie de Trevoux, Lyon.
Google Scholar

Reczyński, W., Muszyńska, B., Opoka, W., Smalec, A. & Sułkowska-Ziaja, K. 2013. Comparative study of metals accumulation in cultured in vitro mycelium and natural grown fruiting bodies of Boletus badius and Cantharellus cibarius. Biological Trace Element Research, 153: 355–362.
Google Scholar

Roberts, J. 2008. Vitamin D2 formation from post-harvest UV-B treatment of mushrooms (Agaricus bisporus) and retention during storage. Food Chemistry, 56: 4541–4544.
Google Scholar