Acta Universitatis Lodziensis. Folia Biologica et Oecologica <p style="text-align: justify;"><em>Acta Universitatis Lodziensis. Folia Biologica et Oecologica</em> publishes original and innovative research papers on all aspects of biology and ecology especially in the field of zoology, hydrobiology, botany, microbiology, biochemistry, biophysics, physiology, behaviour, and evolutionary biology. <em>Folia Biologica et Oecologica</em> publishes standard papers but also longer papers such as review articles that sum up the current state of the research on a particular and important topic.</p> <p style="text-align: justify;"><a href=""><em>Acta Universitatis Lodziensis. Folia Biologica et Oecologica </em>on <strong>Digital Commons (Elsevier)</strong></a></p> en-US (Anna Janaszewska) (Firma Magis) Wed, 30 Dec 2020 00:00:00 +0000 OJS 60 Differentiation of Bacillus anthracis and other Bacillus cereus group bacterial strains using multilocus sequence typing method <p>The study describes the preparation of the phylogenetic differentiation of <em>Bacillus cereus </em>strains. The <em>Bacillus cereus </em>group of bacteria is very important for human and animal health. The multilocus sequence typing scheme has been used to present this group of bacteria’s phylogenetic relationship and structure. The MLST system was established using 60 isolates of <em>B. anthracis, B. cereus sensu stricto</em>, <em>B. thuringiensis</em>, and transitional environment strains of <em>Bacillus spp. </em>As a negative control, five strains of <em>B. subtilis </em>and <em>B. megaterium </em>were used. Primers for amplification and sequencing were designed to target highly conserved internal fragment of seven housekeeping genes: <em>glpF</em>, <em>gmk</em>, <em>ilvD</em>, <em>pta</em>, <em>pur</em>, <em>pycA</em>, and <em>tpi</em>. A total of 22 different sequence types (STs) were distinguished. Analysis of the sequence data showed that all of the <em>Bacillus cereus </em>strains are very closely related. The MLST scheme exhibited a high level of resolution that can be used as an excellent tool for studying the phylogenetic relationship, epidemiology, and population structure of the <em>Bacillus cereus </em>group strains. The MLST method additionally allows us to define the phylogenetic relationship between very closely related strains based on a combination of the sequences of all seven alleles fragments and each of them separately. Thus, this genetic investigation tool is very useful in epidemiological investigation of potential military/ bioterrorist use of <em>B. anthracis</em>.</p> Grzegorz Graniak, Alina Olender, Katarzyna Naylor Copyright (c) 2020 Folia Biologica et Oecologica Wed, 30 Dec 2020 00:00:00 +0000 Impaired base excision repair is related to the pathogenesis of non-alcoholic fatty liver disease <p>Non-alcoholic fatty disease (NAFLD) is a liver disorder that affects up to 30% of the population, mainly in Western countries. It is estimated that up to 75% of NAFLD patients will develop a more aggressive form of the disease, non-alcoholic steatohepatitis (NASH). NAFLD can lead to fibrosis and liver failure; however, it is difficult to diagnose NAFLD due to its non-specific symptoms. Unfortunately, there is no treatment available for this disease. The risk factors of NAFLD are obesity and insulin resistance (IR). The molecular factors that seem to play an important role in the pathogenesis of NAFLD are oxidative stress as well as impaired DNA damage repair processes; a great body of evidence confirms an association with the base excision repair (BER) pathway. The activity of BER is decreased in patients with NAFLD and in animal models of this disease. In order to better understand the underlying basis of the disease, knowledge should be broadened in the area of DNA repair in NAFLD.</p> Sylwia Ziółkowska, Piotr Czarny, Janusz Szemraj Copyright (c) 2020 Folia Biologica et Oecologica Wed, 30 Dec 2020 00:00:00 +0000