The role of the cytokinin biosynthesis pathways in the rate of tobacco leaf senescence

Paulina Węzigowska; Magdalena Trojak; Ernest Skowron

doi:10.18778/1730-2366.18.08

Authors

Paulina Węzigowska Jan Kochanowski University of Kielce, Biotechnology, Poland
Magdalena Trojak Jan Kochanowski University of Kielce, Department of Environmental Biology, Poland https://orcid.org/0000-0002-5771-2047
Ernest Skowron Jan Kochanowski University of Kielce, Department of Environmental Biology, Poland https://orcid.org/0000-0001-5330-9122

DOI:

https://doi.org/10.18778/1730-2366.18.08

Keywords:

dark-induced leaf senescence, cytokinin biosynthesis, stay-green phenotype

Abstract

The regulation of leaf senescence depends on endogenous and exogenous factors, among them phytohormones like cytokinins (CKs). CKs are key players regulating the senescing process, as their endogenous concentration is linked to the onset and rate of senescence progression. Thus, this study aimed to identify the relationship between the activity of endogenous CKs biosynthesis pathways - the cytosolic mevalonate (MVA) and the plastid methyl-erythritol phosphate (MEP) and the rate of leaf senescence. To this end, three distinct tobacco (Nicotiana tabacum L.) cultivars – Xanthi, Golden Virginia and Monte Calme Yellow were analysed. The study involved treatment with exogenous CK – benzyladenine – and two different CK synthesis inhibitors: lovastatin and clomazone. The progression of senescence was induced by light deprivation and monitored with chlorophyll level (SPAD), photosynthetic activity (PAM) and changes in the Rubisco protein profile (SDS-PAGE). Analyses showed that the Xanthi cultivar was characterized by delayed onset of senescence and stay-green phenotype, while Golden Virginia, and particularly Monte Calme Yellow showed rapid leaf senescence. The studies provided valuable information regarding the role of MEP and MVA pathway of CK synthesis in the regulation of tobacco leaf senescence.

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References

Añorga, M., Pintado, A., Ramos, C., De Diego, N., Ugena, L., Novák, O., Murillo, J. 2020. Genes ptz and idi, coding for cytokinin biosynthesis enzymes, are essential for tumorigenesis and in planta growth by P. syringae pv. savastanoi NCPPB 3335. Frontiers in Plant Science, 11: 1294.
Google Scholar

Darwish, M., Vidal, V., Lopez-Lauri, F., Alnaser, O., Junglee, S., El Maataoui, M., Sallanon, H. 2015. Tolerance to clomazone herbicide is linked to the state of LHC, PQ-pool and ROS detoxification in tobacco (Nicotiana tabacum L.). Journal of Plant Physiology, 175: 122–130.
Google Scholar

Guo, Y., Ren, G., Zhang, K., Li, Z., Miao, Y., Guo, H. 2021. Leaf senescence: Progression, regulation, and application. Molecular Horticulture, 1(1): 1–25.
Google Scholar

Hönig, M., Plíhalová, L., Husičková, A., Nisler, J., Doležal, K. 2018. Role of cytokinins in senescence, antioxidant defence and photosynthesis. International Journal of Molecular Sciences, 19(12): 4045.
Google Scholar

Janečková, H., Husičková, A., Ferretti, U., Prčina, M., Pilařová, E., Plačková, L., Pospíšil, P., Dolezal, K., Špundová, M. 2018. The interplay between cytokinins and light during senescence in detached Arabidopsis leaves. Plant, Cell & Environment, 41(8): 1870–1885.
Google Scholar

Kobayashi, K., Suzuki, M., Tang, J., Nagata, N., Ohyama, K., Seki, H., Kiuchi, R., Kaneko, Y., Nakazawa, M., Matsui, M., Matsumoto, S., Yoshida, S., Muranaka, T. 2007. Lovastatin insensitive 1, a novel pentatricopeptide repeat protein, is a potential regulatory factor of isoprenoid biosynthesis in Arabidopsis. Plant and Cell Physiology, 48(2): 322–331.
Google Scholar

Li, Y., Chang, D., Zhang, X., Shi, H., Yang, H. 2021. RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency. Scientific Reports, 11(1): 16802.
Google Scholar

Li, Y., Yang, H., Chang, D., Lin, S., Feng, Y., Li, J., Shi, H. 2017. Biochemical, physiological and transcriptomic comparison between burley and flue-cured tobacco seedlings in relation to carbohydrates and nitrate content. Molecules, 22(12): 2126.
Google Scholar

Mayta, M.L., Hajirezaei, M.R., Carrillo, N., Lodeyro, A.F. 2019. Leaf senescence: The chloroplast connection comes of age. Plants, 8(11): 495.
Google Scholar

Skowron, E., Trojak, M. 2021. Effect of exogenously-applied abscisic acid, putrescine and hydrogen peroxide on drought tolerance of barley. Biologia, 76(2): 453–468.
Google Scholar

Sobieszczuk-Nowicka, E., Wrzesiński, T., Bagniewska-Zadworna, A., Kubala, S., Rucińska-Sobkowiak, R., Polcyn, W., Misztal, L., Mattoo, A. K. 2018. Physio-genetic dissection of dark-induced leaf senescence and timing its reversal in barley. Plant Physiology, 178(2): 654–671.
Google Scholar

Thomas, H. 2013. Senescence, ageing and death of the whole plant. New Phytologist, 197(3): 696–711.
Google Scholar

Tsaballa, A., Sarrou, E., Xanthopoulou, A., Tsaliki, E., Kissoudis, C., Karagiannis, E., Michailidis, M., Martens, S., Sperdouli E., Hilioti, Z., Fotopoulos, V., Nianiou-Obeidat, I., Tsaftaris, A., Madesis, P., Kalivas, A., Ganopoulos, I. 2020. Comprehensive approaches reveal key transcripts and metabolites highlighting metabolic diversity among three oriental tobacco varieties. Industrial Crops and Products, 143: 111933.
Google Scholar

Woo, H.R., Kim, H.J., Nam, H.G., Lim, P.O. 2013. Plant leaf senescence and death–regulation by multiple layers of control and implications for aging in general. Journal of Cell Science, 126(21): 4823–4833.
Google Scholar

Zhao, L., Zhang, H., Zhang, B., Bai, X., Zhou, C. 2012. Physiological and Molecular Changes of Detached Wheat Leaves in Responding to Various Treatments. Journal of Integrative Plant Biology, 54(8): 567–576.
Google Scholar