Effect of the solvent on the extraction of polyphenols from distillery stillage and on their antioxidant activity


  • Wioleta Mikucka University of Warmia and Mazury in Olsztyn, Faculty of Geoengineering, Department of Environmental Biotechnology, Sloneczna 45G, 10-709 Olsztyn, Poland https://orcid.org/0000-0002-6676-1166
  • Magdalena Zielińska University of Warmia and Mazury in Olsztyn, Faculty of Geoengineering, Department of Environmental Biotechnology, Sloneczna 45G, 10-709 Olsztyn, Poland https://orcid.org/0000-0003-1902-4515




DPPH assay, Folin-Ciocalteu, phenolic content, methanolic extract, ethanolic extract


The increase in the costs of storage and disposal of post-production residues has resulted in the search for new directions for their recycling, which is closely related to the necessity of protecting the natural environment and promoting a circular economy. Moreover, the apparent interest shown by the food market in raw materials with high antioxidant activity implies an increasing use of by-products. The objective of the study was to determine the effect of the type and concentration of the solvent on the efficiency of extracting polyphenols from distillery stillage as well as their antioxidant activity by using several solvents: methanol:water (70:30 v/v), methanol:water (100:0 v/v), ethanol:water (70:30 v/v) or ethanol:water (100:0 v/v). The DPPH radical method was used to determine the antioxidant activity of the obtained extracts. The normalised variable (NV) and statistical measure (MS) were determined, based on which the effectiveness of the solvents was evaluated. The highest polyphenolic content and the antioxidant activity were obtained by using ethanol:water (70:30 v/v) as a solvent in the extraction of polyphenolic compounds from distillery stillage.


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How to Cite

Mikucka, W., & Zielińska, M. (2021). Effect of the solvent on the extraction of polyphenols from distillery stillage and on their antioxidant activity. Acta Universitatis Lodziensis. Folia Biologica Et Oecologica, 17, 54–62. https://doi.org/10.18778/1730-2366.16.15